HPLC Column, RP PFP, 3um, 2.1mm ID x 150mm Length, 60A. Cogent Brand. 1 EA.
Additional Info:
The 60322‑15P‑2 Cogent RP PFP™ HPLC Column is designed for high‑resolution separation of halogenated, aromatic, and polar compounds, offering enhanced selectivity compared to traditional C18 columns.
This stationary phase features a pentafluorophenyl (PFP) ligand (commonly referred to as “F5”), bonded via a four‑carbon linker to high‑purity silica with full double end‑capping, providing multiple interaction mechanisms including π‑π interactions, dipole interactions, and hydrogen bonding. These mechanisms enable greater selectivity and improved separation of structurally similar compounds.
The 60 Å pore size is optimized for smaller molecules, providing improved efficiency and sharper peak shapes for low‑molecular‑weight analytes.
150 mm Length – High Resolution for LC‑MS & Analytical Methods
The 150 mm length provides enhanced resolving power, making it ideal for complex separations and method refinement.
Higher resolution than 50 mm and 100 mm columns
Improved separation of closely related compounds
Increased peak capacity for complex samples
✅ Use when maximum resolution is required for small molecule analysis
Flow Rate & System Requirements (2.1 mm ID, 3 µm)
⚠️ Requires low‑flow HPLC or LC‑MS system capability
Typical flow rate range: ~0.2–0.4 mL/min
Optimal starting range: ~0.25–0.35 mL/min
Increased backpressure vs 5 µm columns
Compatible with low‑volume LC‑MS detectors
✅ Sample preparation requirement: filter samples using a 0.2 µm syringe filter prior to injection
Why Choose PFP (F5) for Selective Separations
Enhanced Retention for Halogenated Compounds
Strong interaction with fluorinated and halogenated analytes
Improved selectivity vs C18
Multiple Interaction Mechanisms
π‑π interactions for aromatic compounds
Dipole and hydrogen bonding interactions
Greater control during method development
Optimized for Small Molecules (60 Å)
Improved mass transfer and peak shape
Ideal for low‑molecular‑weight analytes
Cogent RP PFP™ Advantages
Fully End‑Capped Surface
Minimizes silanol activity
Improves peak symmetry
High Efficiency (3 µm Particles)
Higher efficiency than 5 µm columns
Sharper peaks and improved resolution
High Purity Silica
Consistent performance and reproducibility
Reliable analytical results
Typical Applications
Halogenated compound analysis
Aromatic compound separations
Pharmaceutical small‑molecule analysis
LC‑MS analytical workflows
USP L43 method applications
Important Usage Notes
Packed and shipped in reversed‑phase solvents
Designed for low‑flow LC‑MS and analytical HPLC systems (2.1 mm ID)
Higher backpressure than larger particle sizes
Use 0.2 µm filtration to maintain column performance